from Chum Salmon

نویسندگان

  • Yoshio KoiDE
  • Toyohiko Noso
چکیده

Cystatin, a cysteine proteinase inhibitor, was isolated from chum salmon (Oncorilynch"s keta) pituitary glands by ion-exchange chromatography on Mono-Q, gel filtration on Superdex 75, and reverse-phase HPLC on an ODS fo1]owing ethanol-ammonium acetate extraction. Salmon pituitary cystatin was equipotent te chicken egg-white cystatin in the papain inhibitory assay. The cystatin consists of 111 amine acid residues with two disulfide linkages formed between 66-75 and 89-109, and has 43% identical sequences with chicken egg-white cystatin with consensus sequences of reactive sites, Cly(4), Gln-X-Val-X-Cly (48-52), and Ile(Val)-Pro-Trp (96-98).

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تاریخ انتشار 2017